The Anthranilate Synthetase-Anthranilate-5-Phosphoribosylpyrophosphate Phosphoribosyltransferase Aggregate
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چکیده
منابع مشابه
Anthranilate synthase-anthranilate 5-phosphoribosyl 1-pyrophosphate phosphoribosyltransferase from Aerobacter aerogenes.
1. Anthranilate synthase and phosphoribosyltransferase from Aerobacter aerogenes purify simultaneously and sediment together on sucrose gradients, showing that they occur as an enzyme aggregate. Both activities of the intact aggregate are subject to inhibition by tryptophan. 2. By using appropriate auxotrophic mutants it was shown that an intact active enzyme aggregate is formed when the compon...
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In the crystal structure of the title compound, C(21)H(25)NO(11), the hexopyranosyl ring adopts a chair conformation and the five substituents are in equatorial positions. An intra-molecular hydrogen bond between the amino group and a neighbouring carbonyl group is found. Two carbonyl groups are disordered and were refined using a split model.
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HO' H /H2 0 \ I dOOH chorismic acid anthranilic acid (Gibson and Gibson, 1964; Somerville, unpublished results). In Escherichia coli the catalytically active species is a protein complex formed by aggregation of the products of the E and D genes of the tryptophan operon (Ito and Yanofsky, 1966). Anthranilate synthetase is one of a class of amidotransferase enzymes where L-glutamine serves as th...
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Abstract: A procedure employed in the purification of anthranilate synhetase of Escherichia coli SJHI is described. The purified anthranilate synthetase appeared to be homogeneous when examined with poliacrylamide gel electrophoresis. Phenly-sepharose CL-4B and Blue dye sepharose were used for purification. A positive correlation was found between purification and ammonium sulfate especially us...
متن کاملMagnesium Anthranilate Dihydrate
The reaction mixture of magnesium chloride with two equivalents of potassium anthranilate [K+ AnthÐ] in water gives on cooling to 0 ∞C a 60% yield of a precipitate which after drying in a vacuum has the composition [Mg(H2O)6](Anth)2 (1). Recrystallization from hot water affords an octahydrate identified as [Mg(H2O)6](Anth)2(H2O)2 (2) in a single crystal X-ray diffraction study. Contrary to find...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1970
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)62924-2